This IgG1 mouse monoclonal antibody against IGF I can detect a concentration of 0.2 ng IGF I/0.2 ml serum with an affinity of 109 M-1. 50% of I125 IGFI are bound at a final dilution of the ascites of 1:20'000. The antibody crossreact 3% with IGF II but not with insulin and can be used for immunopurification of IGF's from human serum. It recognize IGF I of other species with following approximate relative potencies: guinea pig, rabbit, human, rat and mouse =7:4:1:<0.01:
